APS Annual Meeting at Experimental Biology

The Heart in Miniature: Cell to Organoids in a Petri Dish

Featured Topic — Friday, April 30, 2021 — 10:00 AM - 11:30 AM — Virtual Session, Room APS-6
Cardiovascular Section — Chair: Sakthivel Sadayappan — Co-Chair: Michelle Parvatiyar

This featured topic proposes to present the most recent developments in the use of human induced pluripotent stem(iPS) cells differentiated into cardiomyocytes (CM) to fabricate 3-dimensional heart muscle and cardiac organoids (COs).The use of iPSC-CMs as an in vitro research model provides a major advantage over primary cells for use in multipleexperiments. These iPSC-CMs are ideal for use in cardiotoxicity testing, drug screening, drug validation, as well asmetabolism studies and electrophysiology applications. Recent advances in stem cell biology have led to the generationof iPSC-derived Cos, resulting in a valuable approach toward assessing both cell differentiation potential andinteractions between different cell types of the same genetic background. Indeed, for modeling complex disorders withcell non-autonomous phenotype, such as congenital heart defects, COs may be essential. In particular, in vitro cardiacorganogenesis using human iPSCs (hiPSCs) may lead to new opportunities for high-throughput modeling to study thedisease phenotype, determine the disease mechanism, and perform preclinical studies to screen for therapeuticreagents. These COs provide a tool with which to study organ development, cardiac function and cardiac injury.Therefore, the proposed featured topic aims to present recent developments in studying cardiac organoids using hiPSCsfrom patients with various cardiovascular diseases and control subjects. hCOs will be used to determine cardiacdysfunction, define ultrastructural changes at the sarcomere level, including electromechanical transduction at this keysite, and perform preclinical screening with small molecules. The proposed featured topic will address 1) whether theiPSC-CM model is sufficient to study human disease and accelerate aging in vitro to trigger late onset of diseasephenotypes, 2) the challenges in generating organoids in a Petri dish, and 3) whether the data generated aretranslatable to treat human heart disease. We expect that the audience will learn about cell models, experimentaldesigns, various tools, as well as the challenges and opportunities of studying basic cardiac biology and physiology inhealth and disease at the cell and organoid levels, as recent emerging areas.


  • Uncovering Mechanisms Underlying Arrhythmogenic Heart Disease using hiPSC 
    Farah Sheikh — University of California San Diego Medicine

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