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Innovative Imaging Approaches in Gastrointestinal Epithelial Biology

Symposium — Sunday, April 7, 2019 — 3:30 PM - 5:00 PM — Convention Center, Room W309AB
GI and Liver Physiology Section — Chair: Karen Edelblum — Co-Chair:
Cosponsored by AJP - Gastrointestinal and Liver Physiology

Technological advances in tissue processing and live imaging have provided unique insight into the spatiotemporal mechanisms underlying epithelial responses to intestinal injury and infection. The integration of state-of-the-art microscopy along with the rapid evolution of enteroid culture systems and widespread availability of tissue-specific fluorescent reporters has opened up new avenues of investigation in GI biology. Further, these advanced imaging techniques have greatly impacted the resolution by which we can observe biological responses on a molecular, cellular, or whole tissue level. The goal of this session is to present cutting edge research on how new tools for imaging have shed light into complex epithelial biological processes such as ion transport, host defense and stem cell regeneration in response to injury. This will include time-lapse microscopy of calcium signaling pathways in human enteroids (Joseph Hyser, Baylor College of Medicine), intravital microscopy of goblet cell-associated antigen passages (Kathryn Knoop, WashU) and the use of intact cleared tissue to study stem cell clonality in crypt injury and metaplasia (D. Brent Polk, Childrens Hospital of Los Angeles). Although the development of this technology has been ground-breaking in its own right, the focus of this symposium is to highlight how these advanced imaging approaches can be integrated into existing research programs to address specific biological questions and advance the field of GI biology.

Speakers

  • Microbial Exploitation of Host Calcium Signaling Pathways in Human Enteroids
    Joseph Hyser — Molecular Virology and Microbiology, Baylor College of Medicine

  • Intravital Imaging of Goblet Cell-Associated Antigen Passages
    Kathryn Knoop — Division of Gastroenterology, Washington University

  • Visualization of Intestinal Stem Cell Clones in Crypt Repair Using Tissue Transparency
    Brent Polk — Pediatrics, Childrens Hospital of Los Angeles, University of Southern California





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